The appointments were made following an assessment of COVID-related considerations as a result of the changes to the match locations and where the match officials travel from.
South African referee Jaco Peyper will be the man in the middle in two tests, both involving Argentina.
He will take charge of the first Test between Argentina and New Zealand, to be played at Suncorp Stadium in Brisbane on 18 September, and the final match of the tournament on 2 October, when Argentina takes on Australia at the CBUS Super Stadium in Gold Coast.
Pearce (pictured top) will handle the opening test match between Australia and South Africa on 12 September at CBUS Super Stadium in Gold Coast and will take charge of the much anticipated 100th Test between New Zealand and South Africa on 25 September in Townsville.
Carley, who will make his debut in the Rugby Championship, will hold the whistle when the Boks play the Wallabies at Suncorp Stadium in Brisbane on Saturday, 18 September, and again in the final fixture for the Springboks against the All Blacks on 2 October. The test against New Zealand will be played at CBUS Super Stadium in Gold Coast.
Match official appointments:
South Africa v Australia (CBUS Super Stadium, Gold Coast)
Date: Sunday, 12 September
Referee: Luke Pearce (RFU)
Assistant Referee 1: Damon Murphy (RA)
Assistant Referee 2: Jordan Way (RA)
TMO: Matthew Carley (RFU)
Australia v South Africa (Suncorp Stadium, Brisbane)
Date: Saturday, 18 September
Referee: Matthew Carley (RFU)
Assistant Referee 1: Luke Pearce (RFU)
Assistant Referee 2: Graham Cooper (RA)
TMO: Brett Cronan (RA)
New Zealand v South Africa (Queensland Country Bank Stadium, Townsville)
Date: Saturday, 25 September
Referee: Luke Pearce (RFU)
Assistant Referee 1: Jaco Peyper (SARU)
Assistant Referee 2: Angus Gardner (RA)
TMO: Damon Murphy (RA)
South Africa v New Zealand (CBUS Super Stadium, Gold Coast)
Date: Saturday, 2 October
Referee: Matthew Carley (RFU)
Assistant Referee 1: Damon Murphy (RA)
Assistant Referee 2: Angus Gardner (RA)
TMO: James Leckie (RA)